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Protogel vs biorad's a-bis acrylamide - General Lab Techniques
V p = (XV t) / 30 where V p = volume of 30% ProtoGel, X = per - cent monomer desired in gel and V t = total volume of gel casting solution. EXAMPLE: To make 100 mL of a 10% percent monomer gel, the amount of ProtoGel to add is calculated as follows: V p = (10 *100) / 30 = 33.3 2. Add Initiators and Cast Gel For optimal results, degas gel ...